The study detected 11 organophosphorus pesticides in three environment medias：rice, vegetables and meat. We detected the concentration of different organophosphorus pesticides with GC/MS. The detection limit of meat and rice & vegetables were 0.05 μg/kg and 0.1 μg/kg respectively. Recovery results all met measurement requirements. The result showed that the total concentration of 11 organophosphorus pesticides were higher in rice and vegetables than meat revealing that the amount of pesticide residues in rice and vegetable surface were higher than that inside living organisms. Samples collected form Nanjing was with higher concentration than Changzhou. Organophosphorus pesticides in different environmental medias showed a different detection rate revealing that various environmental media have different impact on the content of pesticide residues.