引用本文:赵春霞,厉以强,梅卓.酶底物法检测地表水粪便污染的适用性探讨[J].环境监控与预警,2009,1(2):25-27
ZHAO Chun-xia,LI Yi-qiang,MEI Zhuo-hua.Applicability of Enzyme Substrate Technique to Fecal Contamination Testing in Surface Water[J].Environmental Monitoring and Forewarning,2009,1(2):25-27
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酶底物法检测地表水粪便污染的适用性探讨
赵春霞1, 厉以强2, 梅卓1
1.南京市环境监测中心站,江苏 南京 210013;2.江苏省环境监测中心,江苏 南京 210036
摘要:
为探讨酶底物法检测地表水粪便污染的适用性,采用酶底物法与多管发酵法同步检测了长江南京段、秦淮河、玄武湖等水中大肠菌群。结果显示:在多数情况下,酶底物法检测结果略低于多管发酵法,两种方法没有统计学差异(P〉0.05);与多管发酵法相比,酶底物法检测粪便污染特异性强、时间周期短、二次污染小、简便快速,适于推广应用。
关键词:  酶底物法  粪大肠菌群  地表水
DOI:
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基金项目:江苏省环境监测科研基金资助项目(0702)
Applicability of Enzyme Substrate Technique to Fecal Contamination Testing in Surface Water
ZHAO Chun-xia1, LI Yi-qiang2, MEI Zhuo-hua1
1.Nanjing Environmental Monitoring Central Station, Nanjing, Jiangsu 210013, China;2.Jiangsu Provincial Environmental Monitoring Center, Nanjing, Jiangsu 210036,China
Abstract:
Test was conducted on the samples from Yangtze River,Qinghuai River and Xuanwu Lake using enzyme substrate and multiple - tube fermentation techniques in parallel to look into the applicability of enzyme substrate technique to fecal contamination in surface water. The results indicated that in most cases the coliform values from enzyme substrate technique were lower than that from multiple -tube fermentation technique. However, there was no statistical discrepancy between them( P 〉 0.05 ). Compared with multiple -tube fermentation technique, enzyme substrate technique characterized with a shorter testing period and lesser secondary pollution, has a better specificity and a wide application.
Key words:  enzyme substrate technique  fecal coliforms  surface water