引用本文:李光明.超声提取-高效液相色谱法测定土壤中氨基苯磺酸的方法研究[J].环境监控与预警,2020,12(6):27-31
LI Guang-ming.Study on Ultrasonic Extraction High Performance Liquid Chromatography Method for Determination of Aminobenzene Sulfonic Acids in Soil[J].Environmental Monitoring and Forewarning,2020,12(6):27-31
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超声提取-高效液相色谱法测定土壤中氨基苯磺酸的方法研究
李光明
作者单位
李光明 中检集团理化检测有限公司上海 200436 
摘要:
建立了超声提取-高效液相色谱法测定土壤中氨基苯磺酸的方法。以甲醇/水(V∶V=1∶1)为萃取溶剂,室温下进行超声波提取,通过固相萃取小柱净化后,以带季铵基团的聚乙烯醇离子色谱柱分离,二极管阵列检测器测定。该实验方法具备较好的抗干扰性,方法曲线相关系数r>0.999,取样量为5 g时,对、间、邻氨基苯磺酸的方法检出限分别为0.1,0.2和0.2 mg/kg,加标回收率分别为83.5%~99.5%,83.1%~98.0%和77.8%~92.5%。对5个地区的土壤样品加标回收实验结果表明,该方法适用性较强,可满足实际样品的测定。
关键词:  高效液相色谱  土壤  对氨基苯磺酸  间氨基苯磺酸  邻氨基苯磺酸
DOI:
分类号:X833;O657.7+2
文献标识码:B
基金项目:
Study on Ultrasonic Extraction High Performance Liquid Chromatography Method for Determination of Aminobenzene Sulfonic Acids in Soil
LI Guang-ming
Abstract:
An Ultrasonic Extraction High Performance Liquid Chromatography method for the determination of aminobenzene sulfonic acids in soil sample was established. Using methanol water(V∶V=1∶1) as extraction solvent, ultrasonic extraction was performed at room temperature, sample extract was cleaned up by solid phase extraction cartridge, separated by a polyvinyl alcohol ion chromatographic column bonded with quaternary ammonium group, and analyzed by diode array detector. The method showed good anti interference performance, the linear correlation coefficient r>0.999, and the method detection limit of ρAminobenzene sulfonic acid was 0.1 mg/kg, m Aminobenzene sulfonic acid was 0.2 mg/kg while o Aminobenzene sulfonic acid was 0.2 mg/kg when sample weight was 5 g. The recoveries were 83.5%~99.5%, 83.1%~98.0% and 77.8%~92.5%, respectively. The results of the recovery experiment of soil samples in 5 regions showed that the method has strong applicability and can meet actual needs.
Key words:  HPLC  Soil  ρAminobenzene sulfonic acid  m Aminobenzene sulfonic acid  o Aminobenzene sulfonic acid