Endocrine-disrupting chemicals(EDCs) are a group of exogenous chemicals released into the environment through human activities, which can interfere with the function of the endocrine system in organisms. As a typical alkylphenol endocrine disruptor, 3-isopropylphenol has attracted extensive attention in the academic community due to its wide industrial applications and potential ecological risks. However, its vascular toxicity and underlying mechanisms remain unclear. This study investigates the vascular toxic effects of 3-isopropylphenol using a zebrafish model. We predicted the toxic mechanisms and target sites of 3-isopropylphenol through network toxicology techniques, and verified them using technical methods such as RT-qPCR. The results showed that exposure to 3-isopropylphenol promotes cell apoptosis in zebrafish larvae by inhibiting the JNK signaling pathway, leading to impaired vascular development. The above research results provide an important basis for in-depth understanding of the toxic effects and mechanisms of 3-isopropylphenol on vascular development in zebrafish larvae, and contribute to further assessment of its potential harm to the ecological environment.